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normal ermal fibroblast  (ATCC)


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    ATCC normal ermal fibroblast
    Normal Ermal Fibroblast, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1914 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcs+201+012+fibroblasts/Primary+Dermal+Fibroblast%3B+Normal%2C+Human%2C+Adult/pm42316883-220-35-38
    Average 99 stars, based on 1914 article reviews
    normal ermal fibroblast - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Mutagenesis:

    Article Title: Generation of CRISPR/Cas9-edited human iPSC lines carrying homozygous and heterozygous SAMD9 p.I983S mutations
    Article Snippet: .. In this study, we describe the generation of two homozygous and two heterozygous iPSC lines carrying SAMD9 c.2948T>G, p.I983S mutation previously reported in two patients with severe MIRAGE syndrome CRISPR/Cas9 gene editing system in induced pluripotent stem cells generated from reprogrammed PCS-201-012 fibroblasts purchased from ATCC and reprogrammed in our center [4, 6-8] (Table 1). .. The reprogramming of the parental cells was achieved by infecting the cells with Sendai virus vector encoding four reprogramming factors (L-MYC, OCT4, SOX2, and KLF4) (Invitrogen, Carlsbad, CA) (Table 1).

    CRISPR:

    Article Title: Generation of CRISPR/Cas9-edited human iPSC lines carrying homozygous and heterozygous SAMD9 p.I983S mutations
    Article Snippet: .. In this study, we describe the generation of two homozygous and two heterozygous iPSC lines carrying SAMD9 c.2948T>G, p.I983S mutation previously reported in two patients with severe MIRAGE syndrome CRISPR/Cas9 gene editing system in induced pluripotent stem cells generated from reprogrammed PCS-201-012 fibroblasts purchased from ATCC and reprogrammed in our center [4, 6-8] (Table 1). .. The reprogramming of the parental cells was achieved by infecting the cells with Sendai virus vector encoding four reprogramming factors (L-MYC, OCT4, SOX2, and KLF4) (Invitrogen, Carlsbad, CA) (Table 1).

    Generated:

    Article Title: Generation of CRISPR/Cas9-edited human iPSC lines carrying homozygous and heterozygous SAMD9 p.I983S mutations
    Article Snippet: .. In this study, we describe the generation of two homozygous and two heterozygous iPSC lines carrying SAMD9 c.2948T>G, p.I983S mutation previously reported in two patients with severe MIRAGE syndrome CRISPR/Cas9 gene editing system in induced pluripotent stem cells generated from reprogrammed PCS-201-012 fibroblasts purchased from ATCC and reprogrammed in our center [4, 6-8] (Table 1). .. The reprogramming of the parental cells was achieved by infecting the cells with Sendai virus vector encoding four reprogramming factors (L-MYC, OCT4, SOX2, and KLF4) (Invitrogen, Carlsbad, CA) (Table 1).

    Cell Culture:

    Article Title: Controlled Sr( ii ) ion release from in situ crosslinking electroactive hydrogels with potential for the treatment of infections
    Article Snippet: .. ATCC PSC-500-010 Human Mesenchymal Stem Cells (MSCs) and ATCC PCS-201-012 fibroblasts were cultured with DMEM/F12 media with 10% fetal bovine serum and 1% penicillin–streptomycin addition in a 37 °C incubator with 5% CO 2 until cells reach enough number. ..



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    (A) Schematic depiction of MBV isolation procedure from in vitro dermal <t>fibroblast</t> cultures. (B) Nanoparticle tracking analysis of diluted MBVs (1:10,000) measures particle size distribution and concentration. (C) Transmission electron micrograph of MBVs reveals vesicle-like morphology. (D) Western blot analysis of Alix (∼100kDa), Integrin β1 (∼100-130 kDa), and CD63 (∼35-55 kDa) expression in MBVs and dermal fibroblast cell lysate. (E) Fluorescence and scanning electron microscopy images of fast-relaxing and slow-relaxing alginate hydrogels with and without MBVs. Top: 5kPa hydrogels encapsulating CFSE-labeled MBVs, * denotes region outside of hydrogel. Bottom: 20kPa hydrogels encapsulating unlabeled MBVs.
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    Image Search Results


    (A) Schematic depiction of MBV isolation procedure from in vitro dermal fibroblast cultures. (B) Nanoparticle tracking analysis of diluted MBVs (1:10,000) measures particle size distribution and concentration. (C) Transmission electron micrograph of MBVs reveals vesicle-like morphology. (D) Western blot analysis of Alix (∼100kDa), Integrin β1 (∼100-130 kDa), and CD63 (∼35-55 kDa) expression in MBVs and dermal fibroblast cell lysate. (E) Fluorescence and scanning electron microscopy images of fast-relaxing and slow-relaxing alginate hydrogels with and without MBVs. Top: 5kPa hydrogels encapsulating CFSE-labeled MBVs, * denotes region outside of hydrogel. Bottom: 20kPa hydrogels encapsulating unlabeled MBVs.

    Journal: bioRxiv

    Article Title: Matrix stiffness and stress relaxation regulate matrix-bound nanovesicle release from alginate hydrogels

    doi: 10.64898/2026.05.28.728372

    Figure Lengend Snippet: (A) Schematic depiction of MBV isolation procedure from in vitro dermal fibroblast cultures. (B) Nanoparticle tracking analysis of diluted MBVs (1:10,000) measures particle size distribution and concentration. (C) Transmission electron micrograph of MBVs reveals vesicle-like morphology. (D) Western blot analysis of Alix (∼100kDa), Integrin β1 (∼100-130 kDa), and CD63 (∼35-55 kDa) expression in MBVs and dermal fibroblast cell lysate. (E) Fluorescence and scanning electron microscopy images of fast-relaxing and slow-relaxing alginate hydrogels with and without MBVs. Top: 5kPa hydrogels encapsulating CFSE-labeled MBVs, * denotes region outside of hydrogel. Bottom: 20kPa hydrogels encapsulating unlabeled MBVs.

    Article Snippet: Primary adult human dermal fibroblasts (41-year-old female donor, American Type Culture Collection, PCS-201-012) were acquired at passage 1.

    Techniques: Isolation, In Vitro, Concentration Assay, Transmission Assay, Western Blot, Expressing, Fluorescence, Electron Microscopy, Labeling